WebDec 1, 2024 · However, the most appealing feature of aptamers is that they can be selected in vitro through an iterative process termed SELEX (Systematic Evolution of Ligands by EXponential enrichment) 2, 3,... WebSep 16, 2024 · High-throughput sequencing SELEX (HT-SELEX) is a powerful technique for unbiased determination of preferred target motifs of DNA-binding proteins in vitro. The procedure depends upon selection of DNA binding sites from a random library of oligonucleotides by purifying protein-DNA complexes and amplifying bound DNA using …
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WebNov 12, 2024 · Cell‐SELEX can not only generate aptamers for specific cell isolation/detection, diagnosis, and therapy, but also lead to the discovery of biomarkers and unexpected molecular events. WebJul 1, 2024 · Among all SELEX technology, Capture-SELEX is a variant of the in vitro selection process, which is suitable for isolating aptamers against small molecules. Capture-SELEX library was developed to enable the immobilization of the oligonucleotides instead of the target molecules during the aptamer selection process. WebMay 4, 2024 · HT-SELEX extends the traditional SELEX protocol, aimed at the generation of high-affinity and specificity oligonucleotides known as aptamers against a molecular target of interest, by coupling this technology with HT sequencing. ... of non-affine species while amplifying the remaining pool to form the input to the next cycle as well as the ... kathir college of engineering neelambur